Neu5Ac (blue), 2,7-anhydro-Neu5Ac (orange), or XY intermediate (gray).
B and C, superposition of the 1H,13C HSQC reference spectra of Neu5Ac (blue) and 2,7-anhydro-Neu5Ac (orange) and 1H,13C HSQC reaction mixture at 30 min (where the XY peak is observed to peak, gray).
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